蛋白酶S- 详细介绍:
英文名称:ProteaseSAureus;EndoproteinaseGlu-CfromStaphylococcusaureusV8;V8Protease
其他名称:V8蛋白酶S
CAS号:66676-43-5
级别:BR
来源:StaphaureusV8
Activity:≥500units/mg
活力定义:OneUnitcausesachangeof0.001A280nmperminuteat37℃,pH7.8usingcaseinasthesubstrate
产品描述:ProteaseS.aureusV8(Endoproteinase-Glu-C)specificallycleavespeptidebondsontheCOOH-terminalsideofeitherasparticorglutamicacids.Inthepresenceofammonium,theenzymespecificityislimitedtoglutamicsites.Ithasamolecularweightof27,000daltonsandoptimumpH'sof4.0and7.8withhemoglobinasthesubstrate.ProteaseS.aureusV8isinhibitedbydiisopropylfluorophosphateandmonovalentanionssuchasF-,Cl-,CH3COO-andNO3.EnzymeactivityisdeterminedbythecaseindigestionassaydescribedbyDrapeau(MethodsEnzymol.,45,469,1976).
性状(以下信息仅供参考):粉末。
用途:本品仅供科研,不得用于其它用途
保存:2~8℃